Catechol substrates
Catechol substrates are colourless compounds that following bacterial hydrolysis produce a brown soluble end-point due to a chelate between catechol and ferrous ions that have been incorporated into the media. Catechol substrates are best suited for use in liquid media due to the colour of the catechol-metal chelate diffusing into the surrounding medium. Catechol substrates work equally well under both aerobic and anaerobic conditions.
Catechol beta-D-glucopyranoside (Product code 14059), catechol beta-D-galactopyranoside (Product code 14045) and catechol beta-D-glucuronide CHA salt (Product code 14065) for the detection of glucopyranosidase activity, galactopyranoside activity and glucuronidase activity respectively. Catechol substrates are non-inhibitory and allow strong microbial growth. Catechol substrates and their use are covered by claims in Glycosynth's patents EP3066107 and US9879303.