2,3-Dihydroxynapthalene (DHN Substrates)

Glycosynth have developed a new range of chelating chromogenic substrates for detecting enzyme activity in liquid media. The new, patented, chromogenic substrates are based on the core molecule 2,3-dihydroxynaphthalene (DHN). Colourless DHN-substrates produce purple coloured chelates with ferric ions following bacterial hydrolysis. The substrates can be used under both aerobic and anaerobic conditions, in the presence of iron. The generated purple colour can mask the yellow produced by ONPG cleavage, making DHN-substrates especially suitable for dual chromogenic systems, such as for the detection of E. coli and coliforms. DHN beta-D-glucuronide CHA [Product code 80065] has a higher sensitivity for E.coli compared to the commonly used substrate X beta-D-glucuronide CHA [our product code 70065]. DHN substrates are protected by European Patents EP3066107, EP3066209 and US Patents US9879303, US9938562, US10443084 if appropriate, as indicated under each product.